Two-phase flow patterns in turbulent flow through a dose diffusion pipe

A numerical investigation is carried out for turbulent particle-laden flow through a dose diffusion pipe for a model reactor system. A Lagrangian Stochastic Monte-Carlo particle-tracking approach and the averaged Reynolds equations with a k-e turbulence model, with a two-layer zonal method in the boundary layer, are used for the disperse and continuous phases. The flow patterns coupled with the particle dynamics are predicted. It is observed that the coupling of the continuous phase with the particle dynamics is important in this case. It was found that the geometry of the throat significantly influences the particle distribution, flow patterns and length of the recirculation region. The accuracy of the simulations depends on the numerical prediction and correction of the fluid phase velocity during a characteristic time interval of the particles. A numerical solution strategy for the computation of two-way momentum coupled flow is discussed. The three test cases show different flow features in the formation of a recirculation region behind the throat. The method will be useful for the qualitative analysis of conceptual designs and their optimisation

Numerical and Experimental Investigation of the Morphology Development of Expansion Clouds by a Powder Jet Flow

Explosion suppression is often the preferred method of explosion attenuation in industry. The morphology development of suppression clouds is important for the design of necessary coverage of the product. This paper presents a numerical and experimental investigation of the growth of powder dispersion as it expands from a discharge nozzle. A Lagrangian stochastic particle-tracking approach and the RNG k-e turbulence model are adopted in the flow field solver for the dispersed and continuous phases. The flow fields coupled with the particle interactions are predicted. The dispersion characteristics of the expansion of the powder cloud through a pipe for short intervals of time are investigated. This was compared with (1) captured images from experiments, (2) experimental data, and (3) results of previous simulations. Particle positions along the jet are presented. The effects of flow rate on the development of the cloud and a comparison with experimental results are also presented. It is noted that the coverage of the powder cloud can be controlled by the flow rate of the jet, and the developing length of the cloud is more influenced by the flow rate of jet flow than the developing width. The good qualitative agreements achieved are useful for further optimisation of product design

COCO_TS Dataset: Pixel-level Annotations Based on Weak Supervision for Scene Text Segmentation

The absence of large scale datasets with pixel-level supervisions is a significant obstacle for the training of deep convolutional networks for scene text segmentation. For this reason, synthetic data generation is normally employed to enlarge the training dataset. Nonetheless, synthetic data cannot reproduce the complexity and variability of natural images. In this paper, a weakly supervised learning approach is used to reduce the shift between training on real and synthetic data. Pixel-level supervisions for a text detection dataset (i.e. where only bounding-box annotations are available) are generated. In particular, the COCO-Text-Segmentation (COCO_TS) dataset, which provides pixel-level supervisions for the COCO-Text dataset, is created and released. The generated annotations are used to train a deep convolutional neural network for semantic segmentation. Experiments show that the proposed dataset can be used instead of synthetic data, allowing us to use only a fraction of the training samples and significantly improving the performances

The role of SARS-CoV-2 aerosol transmission during the COVID-19 pandemic

The COVID-19 pandemic, caused by the virus SARS-CoV-2, has touched most parts of the world and devastated the lives of many. The high transmissibility coupled with the initial poor outcome for the elderly led to crushingly high fatalities. The scientific response to the pandemic has been formidable, aided by advancements in virology, computing, data analysis, instrumentation, diagnostics, engineering and infection control. This has led to improvements in understanding and has helped to challenge some established orthodoxies. Sufficient time has elapsed since the start of the COVID-19 pandemic that a clearer view has emerged about transmission and infection risks, public health responses and related societal and economic impacts. This timely volume has provided an opportunity for the science community to report on these new developments

Loss of APD1 in Yeast Confers Hydroxyurea Sensitivity Suppressed by Yap1p Transcription Factor

Ferredoxins are iron-sulfur proteins that play important roles in electron transport and redox homeostasis. Yeast Apd1p is a novel member of the family of thioredoxin-like ferredoxins. In this study, we characterized the hydroxyurea (HU)-hypersensitive phenotype of apd1Δ cells. HU is an inhibitor of DNA synthesis, a cellular stressor and an anticancer agent. Although the loss of APD1 did not influence cell proliferation or cell cycle progression, it resulted in HU sensitivity. This sensitivity was reverted in the presence of antioxidant N-acetyl-cysteine, implicating a role for intracellular redox. Mutation of the iron-binding motifs in Apd1p abrogated its ability to rescue HU sensitivity in apd1Δ cells. The iron-binding activity of Apd1p was verified by a color assay. By mass spectrometry two irons were found to be incorporated into one Apd1p protein molecule. Surprisingly, ribonucleotide reductase genes were not induced in apd1Δ cells and the HU sensitivity was unaffected when dNTP production was boosted. A suppressor screen was performed and the expression of stress-regulated transcription factor Yap1p was found to effectively rescue the HU sensitivity in apd1Δ cells. Taken together, our work identified Apd1p as a new ferredoxin which serves critical roles in cellular defense against HU.published_or_final_versio

Visual Person Understanding through Multi-Task and Multi-Dataset Learning

We address the problem of learning a single model for person re-identification, attribute classification, body part segmentation, and pose estimation. With predictions for these tasks we gain a more holistic understanding of persons, which is valuable for many applications. This is a classical multi-task learning problem. However, no dataset exists that these tasks could be jointly learned from. Hence several datasets need to be combined during training, which in other contexts has often led to reduced performance in the past. We extensively evaluate how the different task and datasets influence each other and how different degrees of parameter sharing between the tasks affect performance. Our final model matches or outperforms its single-task counterparts without creating significant computational overhead, rendering it highly interesting for resource-constrained scenarios such as mobile robotics

Digital PCR methods improve detection sensitivity and measurement precision of low abundance mtDNA deletions

Mitochondrial DNA (mtDNA) mutations are a common cause of primary mitochondrial disorders, and have also been implicated in a broad collection of conditions, including aging, neurodegeneration, and cancer. Prevalent among these pathogenic variants are mtDNA deletions, which show a strong bias for the loss of sequence in the major arc between, but not including, the heavy and light strand origins of replication. Because individual mtDNA deletions can accumulate focally, occur with multiple mixed breakpoints, and in the presence of normal mtDNA sequences, methods that detect broad-spectrum mutations with enhanced sensitivity and limited costs have both research and clinical applications. In this study, we evaluated semi-quantitative and digital PCR-based methods of mtDNA deletion detection using double-stranded reference templates or biological samples. Our aim was to describe key experimental assay parameters that will enable the analysis of low levels or small differences in mtDNA deletion load during disease progression, with limited false-positive detection. We determined that the digital PCR method significantly improved mtDNA deletion detection sensitivity through absolute quantitation, improved precision and reduced assay standard error

Intrinsic activity in the fly brain gates visual information during behavioral choices

The small insect brain is often described as an input/output system that executes reflex-like behaviors. It can also initiate neural activity and behaviors intrinsically, seen as spontaneous behaviors, different arousal states and sleep. However, less is known about how intrinsic activity in neural circuits affects sensory information processing in the insect brain and variability in behavior. Here, by simultaneously monitoring Drosophila's behavioral choices and brain activity in a flight simulator system, we identify intrinsic activity that is associated with the act of selecting between visual stimuli. We recorded neural output (multiunit action potentials and local field potentials) in the left and right optic lobes of a tethered flying Drosophila, while its attempts to follow visual motion (yaw torque) were measured by a torque meter. We show that when facing competing motion stimuli on its left and right, Drosophila typically generate large torque responses that flip from side to side. The delayed onset (0.1-1 s) and spontaneous switch-like dynamics of these responses, and the fact that the flies sometimes oppose the stimuli by flying straight, make this behavior different from the classic steering reflexes. Drosophila, thus, seem to choose one stimulus at a time and attempt to rotate toward its direction. With this behavior, the neural output of the optic lobes alternates; being augmented on the side chosen for body rotation and suppressed on the opposite side, even though the visual input to the fly eyes stays the same. Thus, the flow of information from the fly eyes is gated intrinsically. Such modulation can be noise-induced or intentional; with one possibility being that the fly brain highlights chosen information while ignoring the irrelevant, similar to what we know to occur in higher animals